Alternative primers to identify a range of apicomplexan parasites

J Microbiol Methods. 2020 Aug:175:105985. doi: 10.1016/j.mimet.2020.105985. Epub 2020 Jun 17.

Abstract

Here we present an alternative polymerase chain reaction (PCR) approach using 18S rDNA to identify apicomplexan parasites. A new primer set was designed and evaluated in silico and in vitro. This new PCR could detect some apicomplexan genera based on amplicon size and identify at least 45 species after sequencing.

Keywords: 18S rRNA; Metagenomics; Molecular detection; Neospora; Plasmodium; Toxoplasma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Primers*
  • DNA, Protozoan / genetics
  • Humans
  • Mice
  • Parasites / isolation & purification*
  • Parasitic Diseases / diagnosis*
  • Polymerase Chain Reaction / methods*
  • RAW 264.7 Cells
  • RNA, Ribosomal, 18S / genetics

Substances

  • DNA Primers
  • DNA, Protozoan
  • RNA, Ribosomal, 18S