Diaphorina citri reovirus is most closely related to fijiviruses

Qian Chen; Shahideh Nouri; Yuele Zhang; Anna Erickson; Bryce W Falk

doi:10.1016/j.virol.2020.04.012

Diaphorina citri reovirus is most closely related to fijiviruses

Virology. 2020 Aug:547:20-26. doi: 10.1016/j.virol.2020.04.012. Epub 2020 May 15.

Authors

Qian Chen¹, Shahideh Nouri², Yuele Zhang³, Anna Erickson⁴, Bryce W Falk⁵

Affiliations

¹ Department of Plant Pathology, University of California, Davis, CA, USA; Institute of Plant Virology, Vector-borne Virus Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, PR China.
² Department of Plant Pathology, University of California, Davis, CA, USA; Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
³ Institute of Plant Virology, Vector-borne Virus Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, PR China.
⁴ Department of Plant Pathology, University of California, Davis, CA, USA.
⁵ Department of Plant Pathology, University of California, Davis, CA, USA. Electronic address: bwfalk@ucdavis.edu.

PMID: 32560901
DOI: 10.1016/j.virol.2020.04.012

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama, is an important insect vector of Candidatus Liberibacter asiaticus, the causal agent of Huanglongbing, which is the most destructive disease of citrus worldwide. Sequences for putative Diaphorina citri reovirus (DcRV) were identified from some worldwide populations of D. citri. Here, field surveys indicated that the virus was common in D. citri populations from Hawaii and Fuzhou of PR China. Electron microscopy showed that DcRV virions possessed a typical reovirus-like morphology. The U. S. and Chinese DcRV isolates both showed 10 segments of double-stranded RNA sharing >96% nucleotide sequence identity, and encoding 11 deduced proteins. All genome segments contained conserved 5' and 3' terminal nucleotide sequences and inverted repeats that are hallmarks of reovirus sequence. Phylogenetic analysis showed that DcRV may be considered a new species of the genus Fijivirus sharing a most recent common ancestor with the insect-specific fijivirus Nilaparvata lugens reovirus.

Keywords: Diaphorina citri reovirus; Fijivirus; Reovirus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

China
Citrus / virology
Open Reading Frames
Phylogeny
Plant Diseases / virology
Reoviridae / classification*
Reoviridae / genetics
Reoviridae / isolation & purification*
Reoviridae / ultrastructure