Early Cellular Responses Induced by Sedimentary Calcite-Processed Particles in Bright Yellow 2 Tobacco Cultured Cells

Daniel Tran; Tingting Zhao; Delphine Arbelet-Bonnin; Takashi Kadono; Patrice Meimoun; Sylvie Cangémi; Camille Noûs; Tomonori Kawano; Rafik Errakhi; François Bouteau

doi:10.3390/ijms21124279

Early Cellular Responses Induced by Sedimentary Calcite-Processed Particles in Bright Yellow 2 Tobacco Cultured Cells

Int J Mol Sci. 2020 Jun 16;21(12):4279. doi: 10.3390/ijms21124279.

Authors

Daniel Tran^{1

2}, Tingting Zhao², Delphine Arbelet-Bonnin², Takashi Kadono^{2

3}, Patrice Meimoun², Sylvie Cangémi², Camille Noûs⁴, Tomonori Kawano^{3

5

6

7}, Rafik Errakhi⁸, François Bouteau^{2

5

6}

Affiliations

¹ Agroscope, Institute for Plant Production Systems, 1964 Conthey, Switzerland.
² Laboratoire Interdisciplinaire des Energies de Demain, Université de Paris, 75013 Paris, France.
³ Graduate School of Environmental Engineering, University of Kitakyushu 1-1, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan.
⁴ Cogitamus Laboratory, 75013 Paris, France.
⁵ LINV Kitakyushu Research Center (LINV@Kitakyushu), Kitakyushu 808-0135, Japan.
⁶ International Photosynthesis Industrialization Research Center, The University of Kitakyushu, Kitakyushu 808-0135, Japan.
⁷ Paris Interdisciplinary Energy Research Institute (PIERI), Université de Paris, 75013 Paris, France.
⁸ Eurofins Agriscience Service, Casablanca 20000, Morocco.

Abstract

Calcite processed particles (CaPPs, Megagreen^®) elaborated from sedimentary limestone rock, and finned by tribomecanic process were found to increase photosynthetic CO₂ fixation grapevines and stimulate growth of various cultured plants. Due to their processing, the CaPPs present a jagged shape with some invaginations below the micrometer size. We hypothesised that CaPPs could have a nanoparticle (NP)-like effects on plants. Our data show that CaPPs spontaneously induced reactive oxygen species (ROS) in liquid medium. These ROS could in turn induce well-known cellular events such as increase in cytosolic Ca²⁺, biotic ROS generation and activation of anion channels indicating that these CaPPs could activate various signalling pathways in a NP-like manner.

Keywords: calcite; calcium; cellular signalization; ion channels; reactive oxygen species; tobacco.

MeSH terms

Calcium / metabolism
Calcium Carbonate / pharmacology*
Cells, Cultured
Geologic Sediments / chemistry*
Nanoparticles
Nicotiana / cytology*
Nicotiana / drug effects
Nicotiana / metabolism
Reactive Oxygen Species / metabolism
Signal Transduction
Voltage-Dependent Anion Channels / metabolism

Substances

Reactive Oxygen Species
Voltage-Dependent Anion Channels
Calcium Carbonate
Calcium