Playing Tag with Your Favorite GPCR Using CRISPR

Mizuho Horioka; Thomas Huber; Thomas P Sakmar

doi:10.1016/j.chembiol.2020.06.001

Playing Tag with Your Favorite GPCR Using CRISPR

Cell Chem Biol. 2020 Jun 18;27(6):642-644. doi: 10.1016/j.chembiol.2020.06.001.

Authors

Mizuho Horioka¹, Thomas Huber², Thomas P Sakmar³

Affiliations

¹ Tri-Institutional Program in Chemical Biology, The Rockefeller University, 1230 York Ave., New York, NY, USA; Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, 1230 York Ave., New York, NY, USA.
² Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, 1230 York Ave., New York, NY, USA.
³ Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, 1230 York Ave., New York, NY, USA. Electronic address: sakmar@rockefeller.edu.

PMID: 32559501
DOI: 10.1016/j.chembiol.2020.06.001

Abstract

In this issue of Cell Chemical Biology, White et al. (2020) describe CRISPR/Cas9-mediated tagging of GPCRs and β-arrestin to provide a method to study receptor signaling in cells under conditions of endogenous genetic control. The strategy, when coupled with luminescence reporter and complementation technologies, provides new avenues to study GPCRs.

Publication types

Comment

MeSH terms

Clustered Regularly Interspaced Short Palindromic Repeats*
Receptors, Chemokine*
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism
beta-Arrestins

Substances

Receptors, Chemokine
Receptors, G-Protein-Coupled
beta-Arrestins