Virus-Induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seed Coat: Description of an Effective Procedure Using the transparent testa 2 Gene as a Selectable Marker

Methods Mol Biol. 2020:2172:233-242. doi: 10.1007/978-1-0716-0751-0_17.

Abstract

Virus-induced gene silencing (VIGS) has been successfully applied for functional analysis of genes in many plant species. Many protocols have been established but mainly for gene expression study in vegetative tissue. Here, we present the critical steps of an optimized procedure of VIGS in flax (Linum usitatissimum L.) seed coat using the transparent testa 2 gene as a selectable marker. The present protocol may serve as an effective tool for functional characterization of genes involved in seed coat formation and/or biological functions.

Keywords: Flavonoids; Flax; Functional genetic; Seed coat; Virus-induced gene silencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flavonoids / genetics
  • Flavonoids / metabolism*
  • Flax / genetics
  • Flax / metabolism*
  • Gene Expression Regulation, Plant / genetics
  • Gene Expression Regulation, Plant / physiology
  • Gene Silencing / physiology
  • Seeds / genetics
  • Seeds / metabolism*

Substances

  • Flavonoids