[First results of investigations of SARS-CoV‑2 RNA in human corneal tissue]

Ophthalmologe. 2020 Jul;117(7):615-617. doi: 10.1007/s00347-020-01151-0.
[Article in German]

Abstract

Preliminary investigations of human corneal tissues from coronavirus disease 2019 (COVID-19) cadaveric donors indicated that no severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA is present. Current eye banking guidelines do not recommend any type of routine testing for SARS-CoV‑2 RNA in post-mortem donor tissue. This is partly based on factors that can influence the test results of the reverse transcription polymerase chain reaction (RT-PCR).

Die ersten Untersuchungen von humanen Hornhäuten von COVID‑19(Coronavirus Disease 2019/Coronaviruserkrankung 2019)-Spendern legen nahe, dass keine SARS-CoV-2(„severe acute respiratory syndrome-coronavirus-2“/schweres akutes respiratorisches Syndrom-Coronavirus-2)-RNA vorhanden ist. Derzeit wird nicht empfohlen, eine routinemäßige Testung von postmortalen Spendergeweben auf SARS-CoV-2-RNA durchzuführen. Dies begründet sich u. a. in den Faktoren, die das RT-PCR („reverse transcription polymerase chain reaction“/Reverse-Transkriptase-Polymerasekettenreaktion) Testergebnis beeinflussen können.

Keywords: COVID-19; Cornea; RT-PCR; Tissue donor; Tissue procurement and processing.

Publication types

  • Review

MeSH terms

  • Betacoronavirus / genetics*
  • COVID-19
  • Coronavirus Infections*
  • Humans
  • Pandemics*
  • Pneumonia, Viral*
  • RNA, Viral / genetics*
  • SARS-CoV-2

Substances

  • RNA, Viral