Transcriptomic analysis reveals MAPK signaling pathways affect the autolysis in baker's yeast

Xiao Li; Han Ye; Chao-Qun Xu; Xiang-Ling Shen; Xiao-Long Zhang; Cong Huang; Ben Cheng; Ya-Li Tan; Ze-Tao Xiao; Yu-Peng Pei; Kun Zou

doi:10.1093/femsyr/foaa036

Transcriptomic analysis reveals MAPK signaling pathways affect the autolysis in baker's yeast

FEMS Yeast Res. 2020 Aug 1;20(5):foaa036. doi: 10.1093/femsyr/foaa036.

Authors

Xiao Li^{1

2}, Han Ye¹, Chao-Qun Xu¹, Xiang-Ling Shen¹, Xiao-Long Zhang¹, Cong Huang², Ben Cheng², Ya-Li Tan², Ze-Tao Xiao¹, Yu-Peng Pei¹, Kun Zou¹

Affiliations

¹ China Light Industry Key Laboratory of Yeast Function, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, Hubei 443002, China.
² Hubei Provincial Key Laboratory of Yeast Function, Angel Yeast Company Limited, Yichang, Hubei 443003, China.

PMID: 32556321
DOI: 10.1093/femsyr/foaa036

Abstract

Yeast autolysis refers to the process in which cells degrade and release intracellular contents under specific conditions by endogenous enzymes such as proteases, nucleases and lipid enzymes. Protein-rich baker's yeast is widely used to produce yeast extract in food industry, however, the molecular mechanism related to baker's yeast autolysis is still unclear. In this study, RNA-seq technology and biochemical analysis were performed to analyze the autolysis processes in baker's yeast. The differentially expressed genes (DEGs), 27 autolysis-related euKaryotic Ortholog Groups (KOG) and three types of autolysis-induced Gene Ontology (GO) were identified and analyzed in detail. A total of 143 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways under autolysis were also assigned. Interestingly, the DEGs were significantly enriched in the mitogen-activated protein kinase (MAPK) signaling pathways and metabolic pathways, and key genes MID2, MTL1, SLT2, PTP2, HKR1 and GPD1 may play important roles in autolysis. Further quantitative PCR was performed to verify the expression pattern in baker's yeast autolysis. Together, all these results indicated that MAPK pathways might play an essential role during autolysis process through inhibiting the metabolism and disrupting cell wall in baker's yeast. This result may provide important clues for the in-depth interpretation of the yeast autolysis mechanism.

Keywords: MAPK; RNA-seq; autolysis; baker's yeast; cell wall; metabolism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autolysis*
Genes, Fungal
MAP Kinase Signaling System*
RNA-Seq
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics*
Transcriptome