Difference in BPA uptake between glioma stem-like cells and their cancerous cells

Fumiyo Yoshida; Tadashi Kurita; Keita Endo; Kei Nakai; Makoto Shirakawa; Alexander Zaboronok; Takao Tsurubuchi; Eiichi Ishikawa; Akira Matsumura

doi:10.1016/j.apradiso.2020.109234

Difference in BPA uptake between glioma stem-like cells and their cancerous cells

Appl Radiat Isot. 2020 Oct:164:109234. doi: 10.1016/j.apradiso.2020.109234. Epub 2020 Jun 3.

Authors

Fumiyo Yoshida¹, Tadashi Kurita², Keita Endo³, Kei Nakai⁴, Makoto Shirakawa⁵, Alexander Zaboronok⁶, Takao Tsurubuchi⁷, Eiichi Ishikawa⁸, Akira Matsumura⁹

Affiliations

¹ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: yoshida.fumiyo.ff@u.tsukuba.ac.jp.
² Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan; Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: s1521243@u.tsukuba.ac.jp.
³ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan; Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: s1211746@u.tsukuba.ac.jp.
⁴ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan; Ibaraki Prefectural University of Health Sciences, 4669-2 Ami, Inashiki, Ibaraki, 300-0331, Japan. Electronic address: kenakai-nsu@umin.ac.jp.
⁵ Department of Pharmaceutical Sciences, Fukuyama University, 1 Sanzo, Gakuen-cho, Fukuyama, Hiroshima, 729-0292, Japan. Electronic address: f40072@fukuyama-u.ac.jp.
⁶ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: a.zaboronok@md.tsukuba.ac.jp.
⁷ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: t-tsurubuchi@md.tsukuba.ac.jp.
⁸ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: e-ishikawa@md.tsukuba.ac.jp.
⁹ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. Electronic address: a-matsumur@md.tsukuba.ac.jp.

PMID: 32554123
DOI: 10.1016/j.apradiso.2020.109234

Abstract

Tumor sphere-forming (TS) glioma stem cells and cancerous TS cells were analyzed in vivo and in vitro. The boron concentration in murine TS tumors was higher than normal tissue. The boron concentration at 24 h was 0.80 ± 0.09 μg/10⁷ in the TS cells, and 1.08 ± 0.08 μg/10⁷ in the cancerous cells. The LAT-1 amino-acid transporter positive rate was 35.4% in the TS cells and 100% in the cancerous cells. These results suggested the relation between LAT-1 expression and boronophenylalanine concentration in vitro.

Keywords: Boron neutron capture therapy; Boronophenylalanine; Glioma; Stem cell.

MeSH terms

Animals
Boron Compounds / pharmacokinetics*
Boron Neutron Capture Therapy
Brain Neoplasms / metabolism*
Brain Neoplasms / pathology
Cell Differentiation
Glioma / metabolism*
Glioma / pathology
Large Neutral Amino Acid-Transporter 1 / metabolism
Mice
Neoplastic Stem Cells / metabolism*
Phenylalanine / pharmacokinetics*

Substances

Boron Compounds
Large Neutral Amino Acid-Transporter 1
Phenylalanine