Genomic features and copper biosorption potential of a new Alcanivorax sp. VBW004 isolated from the shallow hydrothermal vent (Azores, Portugal)

Kesava Priyan Ramasamy; Raju Rajasabapathy; Inga Lips; Chellandi Mohandass; Rathinam Arthur James

doi:10.1016/j.ygeno.2020.06.015

Genomic features and copper biosorption potential of a new Alcanivorax sp. VBW004 isolated from the shallow hydrothermal vent (Azores, Portugal)

Genomics. 2020 Sep;112(5):3268-3273. doi: 10.1016/j.ygeno.2020.06.015. Epub 2020 Jun 14.

Authors

Kesava Priyan Ramasamy¹, Raju Rajasabapathy², Inga Lips³, Chellandi Mohandass⁴, Rathinam Arthur James⁵

Affiliations

¹ Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden; Department of Marine Systems, Tallinn University of Technology, Akadeemia Rd. 15A, 12618 Tallinn, Estonia.
² Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India. Electronic address: sabajuly06@gmail.com.
³ Department of Marine Systems, Tallinn University of Technology, Akadeemia Rd. 15A, 12618 Tallinn, Estonia.
⁴ CSIR-National Institute of Oceanography, Regional Centre, Lokhandwala Road, Four Bungalows, Andheri (West), Mumbai 400 053, India.
⁵ Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.

PMID: 32553480
DOI: 10.1016/j.ygeno.2020.06.015

Abstract

A new Alcanivorax sp. VBW004 was isolated from a shallow hydrothermal vent in Azores Island, Portugal. In this study, we determined VBW004 was resistant to copper. This strain showed maximum tolerance of copper concentrations up to 600 μg/mL. Based on 16S rRNA gene sequencing and phylogeny revealed that this strain was more closely related to Alcanivorax borkumensis SK2. We sequenced the genome of this strain that consist of 3.8 Mb size with a G + C content of 58.4 %. In addition, digital DNA-DNA hybridizations (dDDH) and the average nucleotide identities (ANI) analysis between Alcanivorax borkumensis SK2 and Alcanivorax jadensis T9 revealed that Alcanivorax sp. VBW004 belongs to new species. Functional annotation revealed that the genome acquired multiple copper resistance encoding genes that could assist VBW004 to respond to high Cu toxicity. Our results from biosorption analysis presumed that the VBW004 is an ecologically important bacterium that could be useful for copper bioremediation.

Keywords: Alcanivorax; Biosorption; Copper; Hydrothermal vent; Whole-genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcanivoraceae / classification
Alcanivoraceae / genetics
Alcanivoraceae / isolation & purification
Alcanivoraceae / metabolism*
Azores
Copper / metabolism*
Genome, Bacterial
Genomics
Hydrothermal Vents / microbiology*
Molecular Sequence Annotation
Phylogeny

Substances

Copper