Objective: To establish a stable and rapidly rat model acquired aplastic anemia.
Methods: The SD rats were exposed to 137Csγ-ray at 3.5 and 4.0 Gy ( 91 cGy/min), and intraperitoneally injected with CTX at 35 mg/( kg·d) and CHL at 45 mg/( kg·d) in d 4, 6 and 8 after irradiation; the WBC, platelet and reticulocyte counts in peripheral blood, the smears and nucleated cells counts of bone marrow were observed.
Results: The levels of peripheral blood 3-lineage cells of SD rats treated with 137Csγ-irradiation combined with cyclophosphamide and chloramphenicol were significantly reduced, among which white blood cells, platelets and reticulocytes decreased rapidly, and the number of bone marrow nucleated cells decreased significantly; bone marrow pathological sections showed severe reduction of hematopoietic cells, and the non-hematopoietic cells such as fat cells increased, and a serve or lightly reduction of bone marrow cells were found.
Conclusion: The rat model established by 137Csγ-ray irradiation combined with cyclophosphamide and chloramphenicol meets the clinical characteristics of aplastic anemia, and this study provides a stable rat model for the study of new therapeutic drugs for acquired aplastic anemia.
题目: 获得性再生障碍性贫血SD大鼠模型的建立.
目的: 建立成模较快的大鼠获得性再生障碍性贫血稳定模型.
方法: SD大鼠6-8周龄经 3.5和4.0 Gy 137Csγ射线( 91 cGy /min) 照射后，于d 4、6、8腹腔注射环磷酰胺 35 mg/( kg·d) 、氯霉素 45 mg/(kg·d) ，观察其外周血白细胞数、网织红细胞数、骨髓涂片、骨髓病理切片、骨髓有核细胞数量等指标.
结果: 经137Csγ射线照射联合环磷酰胺、氯霉素腹腔注射的SD大鼠外周血三系细胞均显著减少，其中白细胞、血小板、网织红细胞数迅速减少，骨髓有核细胞数明显下降；骨髓病理切片显示，造血细胞重度减少; 脂肪细胞等非造血细胞增多,骨髓增生程度呈减低或重度减低.
结论: 137Csγ射线联合环磷酰胺与氯霉素建立的大鼠模型符合再生障碍性贫血临床特点，为获得性再生障碍性贫血新型治疗药物研究提供了稳定的大鼠模型.