Toxicity of biosynthesized silver nanoparticles to aquatic organisms of different trophic levels

Abstract

Although biosynthesized nanoparticles are regarded as green products, research on their toxicity to aquatic food chains is scarce. Herein, biosynthesized silver nanoparticles (Alcea rosea-silver nanoparticles, AR-AgNPs) were produced by the reaction of Ag ions with leaf extract of herbal plant Alcea rosea. Then, the toxic effects of AR-AgNPs and their precursors such as Ag⁺ ions and coating agent (A. rosea leaf extract) on organisms of different trophic levels of a freshwater food chain were investigated. To the three studied aquatic organisms including phytoplankton (Chlorella vulgaris), zooplankton (Daphnia magna) and fish (Danio rerio), the coating agents of AR-AgNPs showed no toxic effects, and Ag⁺ ions were more toxic in comparison to AR-AgNPs. Further investigations revealed that the release of Ag⁺ ions from AR-AgNPs to the test media were not considerable due to the high stability of AR-AgNPs, thus the toxicity stemmed mainly from the particles of AR-AgNPs in all the three trophic levels. Based on values of 72-h EC₅₀ for C. vulgaris, 48-h LC₅₀ for D. magna and 96-h LC₅₀ for D. rerio, the most sensitive organism to AR-AgNPs exposure was D. magna (the second trophic level).

Keywords: Aquatic organisms; Biosynthesized nanoparticles; Silver nanoparticles; Toxicity; Trophic level.