Hydrogen peroxide-response nanoprobe for CD44-targeted circulating tumor cell detection and H2O2 analysis

Chunting Li; Jiexuan Wang; Xinmiao Lu; Huang Ge; Xin Jin; Qinhua Guan; Yue Su; Ruijun Pan; Peiyong Li; Wei Cai; Xinyuan Zhu

doi:10.1016/j.biomaterials.2020.120071

Hydrogen peroxide-response nanoprobe for CD44-targeted circulating tumor cell detection and H₂O₂ analysis

Biomaterials. 2020 Oct:255:120071. doi: 10.1016/j.biomaterials.2020.120071. Epub 2020 Jun 8.

Authors

Chunting Li¹, Jiexuan Wang², Xinmiao Lu¹, Huang Ge³, Xin Jin³, Qinhua Guan¹, Yue Su³, Ruijun Pan¹, Peiyong Li⁴, Wei Cai⁵, Xinyuan Zhu⁶

Affiliations

¹ Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China.
² Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China; Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
³ School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.
⁴ Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China. Electronic address: peiyli@vip.sina.com.
⁵ Department of General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China; Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China. Electronic address: caiwei@shsmu.edu.cn.
⁶ School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. Electronic address: xyzhu@sjtu.edu.cn.

PMID: 32540754
DOI: 10.1016/j.biomaterials.2020.120071

Abstract

Circulating tumor cells (CTCs) represent the most common way of tumor metastasis and has been considered as a significant index for tumor diagnosis, staging and prognosis. However, CTC detection and analysis are always limited by the scarcity of CTC in the peripheral blood and the interference of blood cells. Therefore, here we presented with a hydrogen peroxide (H₂O₂)-response nanoprobes with CD44-targeted ability to reduce the interference of blood cells and improve the detection efficiency and accuracy and the pancreatic cancer cell was used to evaluate the feasibility of our probe. Shortly, hydrophobic H₂O₂-response naphthalimide-borate fluorophore was introduced onto the hydrophilic hyaluronic acid to form an amphiphilic complex, which could self-assemble into fluorescent nanoprobes in water. Our studies demonstrated that the nanoprobes were not only able to specifically recognize the pancreatic cancer cells with overexpressed CD44 proteins and reduce the influence of white blood cells in the peripheral blood, but also capable of semi-quantifying H₂O₂ content in CTCs, Which could be further used as a significant index for tumor clinical evaluation and therapy.

Keywords: CTCs detection; Fluorescent nanoprobe; Hydrogen peroxide analysis; Pancreatic cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor
Cell Count
Humans
Hyaluronan Receptors
Hydrogen Peroxide
Neoplastic Cells, Circulating*
Pancreatic Neoplasms* / diagnosis
Prognosis

Substances

Biomarkers, Tumor
CD44 protein, human
Hyaluronan Receptors
Hydrogen Peroxide