The anthocyanin biosynthetic pathway regulated by exogenous and endogenous factors through sophisticated networks has been extensively studied in kiwifruit (Actinidia arguta). However, the role of micro RNAs (miRNAs) as regulatory factor in this process is largely unclear. Here, we demonstrate that miR858 is a negative regulator of anthocyanin biosynthesis by repressing the target gene AaMYBC1 in red-colored kiwifruit. Transient co-transformation in Nicotiana benthamiana confirmed that miR858 could target AaMYBC1, which was identified to be an R2R3-type tanscription factor (TF). Subcellular localization showed that AaMYBC1 was located in the nucleus, indicating AaMYBC1 protein could act as a transcriptional regulator in plant cells. Functional protein association network analysis and the yeast two hybrid (Y2H) assay revealed that AaMYBC1 and AabHLH42 interact with each other. Silencing of AaMYBC1 using the virus-induced gene silencing method in the core of A. arguta 'HB' ('Hongbaoshixing', a kind of red-fleshed A. arguta cultivar) fruits reduced the accumulation of anthocyanin and decreased the expression of late biosynthetic genes. miR858 overexpression played a stronger role than AaMYBC1 silencing in the inhibition of coloration. With overexpression of miR858, A. arguta did not present coloration, and anthocyanin was hardly detected. Together, these results clarify the negative regulatory role of miR858 in mediating anthocyanin biosynthesis and accumulation in A. arguta, providing novel insights into the molecular mechanism of anthocyanin biosynthesis.
Keywords: AaMYBC1; Actinidia arguta; Anthocyanin; Overexpression; miR858.
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