TaSPL13 regulates inflorescence architecture and development in transgenic wheat (Triticum aestivum L.)

Li Li; Fu Shi; Yaqiong Wang; Xiaofen Yu; Jingjing Zhi; Yanbin Guan; Hongyan Zhao; Junli Chang; Mingjie Chen; Guangxiao Yang; Yuesheng Wang; Guangyuan He

doi:10.1016/j.plantsci.2020.110516

TaSPL13 regulates inflorescence architecture and development in transgenic wheat (Triticum aestivum L.)

Plant Sci. 2020 Jul:296:110516. doi: 10.1016/j.plantsci.2020.110516. Epub 2020 May 5.

Authors

Li Li¹, Fu Shi², Yaqiong Wang³, Xiaofen Yu⁴, Jingjing Zhi⁵, Yanbin Guan⁶, Hongyan Zhao⁷, Junli Chang⁸, Mingjie Chen⁹, Guangxiao Yang¹⁰, Yuesheng Wang¹¹, Guangyuan He¹²

Affiliations

¹ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: hustll2017@hust.edu.cn.
² The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: hustshifu@hust.edu.cn.
³ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: wangyaqiongyou@163.com.
⁴ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: yuixf@hust.edu.cn.
⁵ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: 1312834905@qq.com.
⁶ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: gybhust@hust.edu.cn.
⁷ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: 1422898789@qq.com.
⁸ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: cjl@hust.edu.cn.
⁹ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: cmj@hust.edu.cn.
¹⁰ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: ygx@hust.edu.cn.
¹¹ The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: wysh@hust.edu.cn.
¹² The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China. Electronic address: hegy@hust.edu.cn.

PMID: 32539997
DOI: 10.1016/j.plantsci.2020.110516

Abstract

The SQUAMOSA promoter-binding protein-like (SPL) proteins play vital roles in plant growth and development in rice (Oryza sative L.) and Arabidopsis thaliana (L.) Heynh. However, few studies regarding the SPL proteins have been reported in wheat. In this study, 56 TaSPLs were clustered into eight groups according to an OsSPL phylogenetic comparison analysis. The expression patterns of TaSPLs in different tissues were analysed by RNA-seq data, and partial results were confirmed by qRT-PCR. Based on the above results, genes such as TaSPL13 and TaSPL15 may be involved in spike or seed development in wheat. Multiple genes that regulate the inflorescence architecture of rice have been identified. Additionally, studies on the genes associated with spikelet development in wheat have been reported relatively rarely. Here, TaSPL13-2B was transferred into wheat cv. Bobwhite. Compared with the wild type, the transgenic lines showed significant increases in the number of florets and grains per spike, indicating that TaSPL13-2B could influence the floret development of wheat. TaSPL13-2B was transferred into rice cv. Nipponbare, which demonstrated that TaSPL13-2B can modify panicle architecture in transgenic rice, with significant increases in panicle length, the number and length of primary branches, and the number of secondary branches.

Keywords: Expression profiles; Floret development; SBP-box transcription factors; TaSPL13-2B transgenic wheat lines; Yield.

MeSH terms

Blotting, Southern
Cloning, Molecular
Gene Expression Regulation, Plant / genetics
Inflorescence / anatomy & histology
Inflorescence / growth & development*
Oryza
Phylogeny
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Proteins / physiology*
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Real-Time Polymerase Chain Reaction
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription Factors / physiology*
Transcriptome
Triticum / anatomy & histology
Triticum / genetics
Triticum / growth & development*

Substances

Plant Proteins
Transcription Factors