MiR-520c-3p alleviates LPS-induced A549 cell and mice lung injury via targeting NLRC5

Pharmazie. 2020 Jun 1;75(6):275-278. doi: 10.1692/ph.2020.0355.

Abstract

MicroRNAs (miRNAs) play important roles in the progression of acute lung injury (ALI). So far, little is known about the role of miR-520c-3p in ALI. In this study, the mechanism of the occurrence and development of ALI was explored. We firstly found that miR-520c-3p could inhibit the expression of NLRC5. A549 cells were treated with lipopolysaccharide (LPS) in vitro to simulate ALI cells and inducing ALI model mice. Moreover, miR-520c-3p overexpression enhanced the viability of damaged cells, inhibited LPS-induced apoptosis, and decreased LPS-induced IL-1β, IL-6 and TNF-α. In addition, the NLRC5 was a direct target of miR-520c-3p. And NLRC5 partially aggravated inflammation injury. In conclusion, miR-520c-3p alleviates LPS-induced inflammatory injury via regulating NLRC5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A549 Cells
  • Acute Lung Injury / genetics*
  • Acute Lung Injury / physiopathology
  • Animals
  • Cell Survival / genetics
  • Disease Models, Animal
  • Humans
  • Inflammation / genetics*
  • Inflammation / pathology
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Lipopolysaccharides
  • Male
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Lipopolysaccharides
  • MIRN520 microRNA, human
  • MicroRNAs
  • NLRC5 protein, human
  • NLRC5 protein, mouse