Consumer diet estimation with biotracer-based mixing models provides valuable information about trophic interactions and the dynamics of complex ecosystems. Here, we assessed the performance of four Bayesian and three numerical optimization-based diet estimation methods for estimating the diet composition of herbivorous zooplankton using consumer fatty acid (FA) profiles and resource library consisting of the results of homogeneous diet feeding experiments. The method performance was evaluated in terms of absolute errors, central probability interval checks, the success in identifying the primary resource in the diet, and the ability to detect the absence of resources in the diet. Despite occasional large inconsistencies, all the methods were able to identify the primary resource most of the time. The numerical optimization method QFASA using χ2(QFASA-CS) or Kullback--Leibler (QFASA-KL) distance measures had the smallest absolute errors, most frequently found the primary resource, and adequately detected the absence of resources. While the Bayesian methods usually performed well, some of the methods produced ambiguous results and some had much longer computing times than QFASA. Therefore, we recommend using QFASA-CS or QFASA-KL. Our systematic tests showed that FA models can be used to accurately estimate complex dietary mixtures in herbivorous zooplankton. This article is part of the theme issue 'The next horizons for lipids as 'trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.
Keywords: Daphnia; FASTAR; MixSIAR; QFASA; biotracers; food web.