Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury

Genomics. 2020 Sep;112(5):3315-3321. doi: 10.1016/j.ygeno.2020.06.018. Epub 2020 Jun 11.

Abstract

Aim: The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control.

Method: The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord injury, 41 individuals with chronic spinal cord injury, and 36 healthy control using real-time reverse-transcription polymerase chain reaction method.

Results: The results showed that lncRNA-TSIX and the TP53INP2 mRNA expression levels in SCI patients was overexpressed in comparison to the control group alongside with a significant downregulation of miR-1283. Statistically,there was a highly significant positive correlation between lnc-RNA-TRIX and TP53INP2 mRNA with inverse correlation between miRNA-1283 and lnc-RNA-TRIX based on fold changes.

Conclusion: Up-regulation of lncRNA-TSIX, TP53INP2 mRNA with downregulation of miRNA-1283 might be closely associated with progression of SCI.

Keywords: Serum; Spinal cord injury; lncRNA; mRNA; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Female
  • Gene Expression Regulation
  • Humans
  • Male
  • MicroRNAs / blood*
  • Middle Aged
  • Nuclear Proteins / blood
  • Nuclear Proteins / genetics*
  • Pilot Projects
  • RNA, Long Noncoding / blood*
  • RNA, Messenger / blood
  • Spinal Cord Injuries / blood
  • Spinal Cord Injuries / diagnosis
  • Spinal Cord Injuries / genetics*
  • Spinal Cord Injuries / pathology

Substances

  • MIRN1283 microRNA, human
  • MicroRNAs
  • Nuclear Proteins
  • RNA, Long Noncoding
  • RNA, Messenger
  • TP53INP2 protein, human
  • TSIX long non-coding RNA, human