Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Aurileide M B F Soares; Lizia M O Gonçalves; Ruanna D S Ferreira; Jefferson M de Souza; Raul Fangueiro; Michel M M Alves; Fernando A A Carvalho; Anderson N Mendes; Welter Cantanhêde

doi:10.1016/j.carbpol.2020.116498

Immobilization of papain enzyme on a hybrid support containing zinc oxide nanoparticles and chitosan for clinical applications

Carbohydr Polym. 2020 Sep 1:243:116498. doi: 10.1016/j.carbpol.2020.116498. Epub 2020 May 26.

Authors

Aurileide M B F Soares¹, Lizia M O Gonçalves¹, Ruanna D S Ferreira², Jefferson M de Souza³, Raul Fangueiro⁴, Michel M M Alves⁵, Fernando A A Carvalho⁶, Anderson N Mendes², Welter Cantanhêde⁷

Affiliations

¹ Departament of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil.
² Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Piauí, Brazil.
³ Department of Fashion Design, Federal University of Piauí, Teresina, Piauí, Brazil.
⁴ Center for Textile Science and Technology, University of Minho, Guimarães, Portugal.
⁵ Department of Veterinary Morphophysiology, Federal University of Piauí, Teresina, Piauí, Brazil.
⁶ Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, Piauí, Brazil.
⁷ Departament of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil. Electronic address: welter@ufpi.edu.br.

PMID: 32532402
DOI: 10.1016/j.carbpol.2020.116498

Abstract

A new hybrid bionanomaterial composed of zinc oxide nanoparticles (ZnO NPs) and chitosan was constructed after enzymatic immobilization of papain for biomedical applications. In this work, we report the preparation and characterization steps of this bionanomaterial and its biocompatibility in vitro. The properties of the immobilized papain system were investigated by transmission electron microscopy, zeta potential, DLS, UV-vis absorption spectroscopy, FTIR spectroscopy, and X-ray diffraction. The prepared bionanomaterial exhibited a nanotriangular structure with a size of 150 nm and maintained the proteolytic activity of papain. In vitro analyses demonstrated that the immobilized papain system decreased the activation of phagocytic cells but did not induce toxicity. Based on the results obtained, we suggest that the novel bionanomaterial has great potential in biomedical applications in diseases such as psoriasis and wounds.

Keywords: Chitosan; Clinical application; Enzyme immobilization; Hybrid materials; Papain; Zinc oxide.

MeSH terms

Animals
Biocompatible Materials* / chemical synthesis
Biocompatible Materials* / chemistry
Cells, Cultured
Chitosan / chemistry*
Enzymes, Immobilized / chemistry
Female
Male
Mice
Mice, Inbred BALB C
Nanoparticles / chemistry*
Papain / chemistry*
Zinc Oxide / chemistry*

Substances

Biocompatible Materials
Enzymes, Immobilized
Chitosan
Papain
Zinc Oxide