Cellular toxicological characterization of a thioxolated arsenic-containing hydrocarbon

Franziska Ebert; Vanessa Ziemann; Viktoria Klara Wandt; Barbara Witt; Sandra Marie Müller; Nikolaus Guttenberger; Ezgi Eyluel Bankoglu; Helga Stopper; Georg Raber; Kevin A Francesconi; Tanja Schwerdtle

doi:10.1016/j.jtemb.2020.126563

Cellular toxicological characterization of a thioxolated arsenic-containing hydrocarbon

J Trace Elem Med Biol. 2020 May 24:61:126563. doi: 10.1016/j.jtemb.2020.126563. Online ahead of print.

Authors

Affiliations

¹ University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany. Electronic address: fraebert@uni-potsdam.de.
² University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany. Electronic address: vziemann@uni-potsdam.de.
³ University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany. Electronic address: vwandt@uni-potsdam.de.
⁴ University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany. Electronic address: bwitt@uni-potsdam.de.
⁵ University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany.
⁶ University of Graz, Institute of Chemistry, NAWI Graz, Universitaetsplatz 1, Graz, Austria.
⁷ University of Würzburg, Institute of Pharmacology and Toxicology, Department of Toxicology, Versbacher Str. 9, Würzburg, Germany. Electronic address: ezgi.bankoglu@uni-wuerzburg.de.
⁸ University of Würzburg, Institute of Pharmacology and Toxicology, Department of Toxicology, Versbacher Str. 9, Würzburg, Germany. Electronic address: stopper@toxi.uni-wuerzburg.de.
⁹ University of Graz, Institute of Chemistry, NAWI Graz, Universitaetsplatz 1, Graz, Austria. Electronic address: georg.raber@uni-graz.at.
¹⁰ University of Graz, Institute of Chemistry, NAWI Graz, Universitaetsplatz 1, Graz, Austria. Electronic address: kevin.francesconi@uni-graz.at.
¹¹ University of Potsdam, Institute of Nutritional Science, Department of Food Chemistry, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany. Electronic address: tanja.schwerdtle@uni-potsdam.de.

PMID: 32531707
DOI: 10.1016/j.jtemb.2020.126563

Abstract

Arsenolipids, especially arsenic-containing hydrocarbons (AsHC), are an emerging class of seafood originating contaminants. Here we toxicologically characterize a recently identified oxo-AsHC 332 metabolite, thioxo-AsHC 348 in cultured human liver (HepG2) cells. Compared to results of previous studies of the parent compound oxo-AsHC 332, thioxo-AsHC 348 substantially affected cell viability in the same concentration range but exerted about 10-fold lower cellular bioavailability. Similar to oxo-AsHC 332, thioxo-AsHC 348 did not substantially induce oxidative stress nor DNA damage. Moreover, in contrast to oxo-AsHC 332 mitochondria seem not to be a primary subcellular toxicity target for thioxo-AsHC 348. This study indicates that thioxo-AsHC 348 is at least as toxic as its parent compound oxo-AsHC 332 but very likely acts via a different mode of toxic action, which still needs to be identified.

Keywords: Arsenic-containing hydrocarbon; Arsenolipids; Genotoxicity; Liver cells; Oxidative stress; thioxo-AsHC 348.

Grants and funding

I 2412/FWF_/Austrian Science Fund FWF/Austria