Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Seung Hee Baek; Hyun Woo Song; Sunwoong Lee; Jung-Eun Kim; Yeo Hyang Kim; Jung-Sub Wi; Jong G Ok; Jun Seok Park; Seonki Hong; Moon Kyu Kwak; Hye Jin Lee; Sung-Wook Nam

doi:10.3389/fchem.2020.00285

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Front Chem. 2020 May 26:8:285. doi: 10.3389/fchem.2020.00285. eCollection 2020.

Authors

Affiliations

¹ Department of Molecular Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea.
² Department of Mechanical Engineering, School of Mechanical Engineering, Kyungpook National University, Daegu, South Korea.
³ Department of Pediatrics, School of Medicine, Kyungpook National University, Daegu, South Korea.
⁴ Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Daejeon, South Korea.
⁵ Department of Mechanical and Automotive Engineering, Seoul National University of Science and Technology, Seoul, South Korea.
⁶ Department of Surgery, School of Medicine, Kyungpook National University, Daegu, South Korea.
⁷ Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, South Korea.
⁸ Department of Chemistry and Green Nano Materials Research Center, Kyungpook National University, Daegu, South Korea.

Abstract

Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal.

Keywords: Au LSPR strip; Au nanocube; IL-10; nanoarchitecture; roll-to-roll.