Abstract
The leaves of the kaffir lime (Citrus hystrix) are commonly used in cuisine and folk medicine. The aim of this study was to isolate a bioactive compound in kaffir lime leaves and characterize its biological activity. The compound was isolated from a hexane fractional extract and identified as agrostophillinol. This is the first report of agrostophillinol isolated from kaffir lime leaves. In terms of cytotoxicity, agrostophillinol exhibited IC50 values of 36.27 ± 7.30 and 53.44 ± 10.63 μg/mL against EoL-1 and HL60 cells, respectively. Agrostophillinol also exhibited potent anti-inflammatory activity, significantly inhibiting IL-6 secretion.
Keywords:
Agrostophillinol; Anti-inflammatory; Anticancer; Antioxidant; Citrus hystrix; Kaffir lime.
Copyright © 2020 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Biphenyl Compounds / antagonists & inhibitors
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Citrus / chemistry*
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Humans
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Interleukin-6 / antagonists & inhibitors*
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Interleukin-6 / metabolism
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Lanosterol / analogs & derivatives*
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Lanosterol / chemistry
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Lanosterol / isolation & purification
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Lanosterol / pharmacology
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Mice
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Molecular Conformation
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Picrates / antagonists & inhibitors
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Leaves / chemistry
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RAW 264.7 Cells
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Structure-Activity Relationship
Substances
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24-methylenelanosta-9(11)-en-3beta-ol
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Anti-Inflammatory Agents
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Antineoplastic Agents, Phytogenic
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Antioxidants
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Biphenyl Compounds
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IL6 protein, human
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Interleukin-6
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Picrates
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Plant Extracts
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Lanosterol
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1,1-diphenyl-2-picrylhydrazyl