Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde

Haodao Mo; Jianhui Qiu; Chao Yang; Limin Zang; Eiichi Sakai; Jiao Chen

doi:10.1016/j.enzmictec.2020.109561

Porous biochar/chitosan composites for high performance cellulase immobilization by glutaraldehyde

Enzyme Microb Technol. 2020 Aug:138:109561. doi: 10.1016/j.enzmictec.2020.109561. Epub 2020 Apr 21.

Authors

Haodao Mo¹, Jianhui Qiu², Chao Yang³, Limin Zang³, Eiichi Sakai¹, Jiao Chen⁴

Affiliations

¹ Department of Machine Intelligence and Systems Engineering, Faculty of Systems Science and Technology, Akita Prefectural University, Akita 015-0055, Japan.
² Department of Machine Intelligence and Systems Engineering, Faculty of Systems Science and Technology, Akita Prefectural University, Akita 015-0055, Japan. Electronic address: qiu@akita-pu.ac.jp.
³ College of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, China.
⁴ Morning Tech Corporation, Shanghai 202103, China.

PMID: 32527530
DOI: 10.1016/j.enzmictec.2020.109561

Abstract

In this work, porous biochar derived from sugarcane bagasse was prepared and then coated with different amounts of chitosan (C@CS) for cellulase immobilization. Cellulase was covalently immobilized on the support by using glutaraldehyde as a linker. The chemical characteristics and morphology of the samples were determined by SEM, BET, FT-IR and XPS. The properties of immobilized enzyme were evaluated by activity recovery, optimum pH value and temperature, and recyclability. The results showed that all the three kinds of immobilized cellulase did not change the optimum pH value of 4 and temperature of 60 °C, and they also exhibited good activity and reusability. Especially for C@CS25 (the feeding ratio of porous biochar to chitosan was 0.5 g: 25 mg), the support retained the morphology of porous biochar well. The corresponding immobilized cellulase kept 67 % activity of free cellulase at pH = 4 and 60 °C, and showed a glucose productivity of 90.8 % even after 10 cycles.

Keywords: Cellulase immobilization; Chitosan; Covalent bond; Porous biochar.

MeSH terms

Cellulase / chemistry*
Cellulase / metabolism
Cellulose / chemistry
Charcoal / chemistry*
Chitosan / chemistry*
Enzyme Stability
Enzymes, Immobilized / chemistry*
Enzymes, Immobilized / metabolism
Glucose / metabolism
Glutaral / chemistry*
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Porosity
Saccharum / chemistry
Temperature

Substances

Enzymes, Immobilized
biochar
Charcoal
Cellulose
bagasse
Chitosan
Cellulase
Glucose
Glutaral