Integrated response of growth, antioxidant defense and isotopic composition to microplastics in juvenile guppy (Poecilia reticulata)

Jun-Nan Huang; Bin Wen; Liu-Jiang Meng; Xin-Xin Li; Mei-Hui Wang; Jian-Zhong Gao; Zai-Zhong Chen

doi:10.1016/j.jhazmat.2020.123044

Integrated response of growth, antioxidant defense and isotopic composition to microplastics in juvenile guppy (Poecilia reticulata)

J Hazard Mater. 2020 Nov 15:399:123044. doi: 10.1016/j.jhazmat.2020.123044. Epub 2020 May 27.

Authors

Jun-Nan Huang¹, Bin Wen², Liu-Jiang Meng¹, Xin-Xin Li¹, Mei-Hui Wang¹, Jian-Zhong Gao¹, Zai-Zhong Chen³

Affiliations

¹ National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
² National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai 201306, China. Electronic address: bwen@shou.edu.cn.
³ National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai 201306, China. Electronic address: chenzz@shou.edu.cn.

PMID: 32521315
DOI: 10.1016/j.jhazmat.2020.123044

Abstract

Microplastics (MPs) pollution becomes a research hotspot and many studies focus on threats of MPs, but few have integrated multi-level indicators to assess response to MPs of organisms. Here we exposed guppy (Poecilia reticulata) to MPs (polystyrene; 32-40 μm diameter) with two concentrations (100 and 1000 μg/L) for 28 days. We found that higher accumulation of MPs appeared in guppy gill than that in gut. MPs had no obvious effect on guppy growth but significantly inhibited the condition factor. Oxidative stress presented in guppy viscera with activated antioxidants. The decline of Na⁺/K⁺-ATP activity in guppy indicated that MPs might interfere with the osmotic balance of gills. MPs reduced body molar ratio of C:N and δ¹³C value, but no apparent impact on δ¹⁵N. It implied that MPs probably altered elemental transition. Eventually, through integrated biomarkers response index (IBR) of guppy, we found that catalase activity was the highest index in response to MPs, and the response of growth performance to MPs was lower than that of oxidative stress and element alteration. Risks of MPs aggravated in a concentration-dependent manner. These findings suggested that multi-level IBR approach should be adopted to quantify effects of MPs on aquatic organisms, especially on fish.

Keywords: IBR; Microparticle; Oxidative stress; Stable isotope.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants
Microplastics*
Oxidative Stress
Plastics
Poecilia*

Substances

Antioxidants
Microplastics
Plastics