Two photoactive Ru (II) compounds based on tetrazole ligands for photodynamic therapy

Jie Yang; Xin He; Zhen Ke; Jianjiao Chen; Zhenyuan Zou; Bo Wei; Dengfeng Zou; Jianhua Zou

doi:10.1016/j.jinorgbio.2020.111127

Two photoactive Ru (II) compounds based on tetrazole ligands for photodynamic therapy

J Inorg Biochem. 2020 Sep:210:111127. doi: 10.1016/j.jinorgbio.2020.111127. Epub 2020 Jun 1.

Authors

Jie Yang¹, Xin He², Zhen Ke³, Jianjiao Chen³, Zhenyuan Zou³, Bo Wei⁴, Dengfeng Zou⁵, Jianhua Zou⁶

Affiliations

¹ School of Pharmacy, Guilin Medical University, Guilin 541004, Guangxi, PR China; Department of Chemistry & Materials Engineering, Jiangsu Key Laboratory of Advanced Functional Materials, Changshu Institute of Technology, Changshu 215500, PR China.
² College of Chemical Engineering, State Key Laboratory of Material-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.
³ School of Pharmacy, Guilin Medical University, Guilin 541004, Guangxi, PR China.
⁴ Department of Chemistry & Materials Engineering, Jiangsu Key Laboratory of Advanced Functional Materials, Changshu Institute of Technology, Changshu 215500, PR China.
⁵ School of Pharmacy, Guilin Medical University, Guilin 541004, Guangxi, PR China. Electronic address: zdf1226@126.com.
⁶ Department of Chemistry & Materials Engineering, Jiangsu Key Laboratory of Advanced Functional Materials, Changshu Institute of Technology, Changshu 215500, PR China. Electronic address: zoujh93@126.com.

PMID: 32521288
DOI: 10.1016/j.jinorgbio.2020.111127

Abstract

Ru (II) compounds have potential application in photodynamic therapy (PDT). In the current study, two Ru (II) compounds based on the auxiliary ligand 2,2'-bipyridine (bipy) by changing main ligands 5-(2-pyridyl) tetrazole (Hpytz) and di(2H-tetrazol-5-yl) amine (H₂datz) have been successfully synthesized and characterized, [Ru (pytz)(bipy)₂][PF₆] (1) and [Ru(Hdatz)(bipy)₂][PF₆] (2). These compounds can form nanoparticles (NPs) by nano-precipitation. And [Ru(pytz)(bipy)₂][PF₆] NPs with a lower half maximal inhibitory concentration (IC₅₀) of 37 μg/mL on HeLa cells than that of [Ru(Hdatz)(bipy)₂][PF₆] NPs (65 μg/mL). Meanwhile, negligible dark toxicity has been also observed for these NPs even under high concentrations. The results show that [Ru(pytz)(bipy)₂][PF₆] (1) and [Ru(Hdatz)(bipy)₂][PF₆] (2) NPs can inhibit cell proliferation in vitro, and may be potential candidates for photodynamic therapy.

Keywords: HeLa; Photodynamic therapy; Ru(II); Tetrazole.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Antineoplastic Agents / radiation effects
Cell Movement / drug effects
Coordination Complexes / chemistry
Coordination Complexes / pharmacology*
Coordination Complexes / radiation effects
Fluorescence
Fluorescent Dyes / chemistry
Fluorescent Dyes / pharmacology
Fluorescent Dyes / radiation effects
HeLa Cells
Humans
Ligands
Light
Nanoparticles / chemistry
Nanoparticles / radiation effects
Photosensitizing Agents / chemistry
Photosensitizing Agents / pharmacology*
Photosensitizing Agents / radiation effects
Ruthenium / chemistry
Ruthenium / radiation effects
Singlet Oxygen / metabolism
Tetrazoles / chemistry
Tetrazoles / pharmacology*
Tetrazoles / radiation effects

Substances

Antineoplastic Agents
Coordination Complexes
Fluorescent Dyes
Ligands
Photosensitizing Agents
Tetrazoles
Singlet Oxygen
Ruthenium