Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number

Abstract

Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (Re_L) and the k_La coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVE^TM 25 system, on reached values of the apparent maximal specific growth rate (μ_max) and the specific yield of biomass (Y^*_X/S) has been identified. The values of Re_L (i.e., 510-10,208), as well as k_La coefficient (i.e., 2.83-13.55 h^-1), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μ_max = 0.038 h^-1 and Y^*_X/S = 25.64 × 10⁸ cells g_glc^-1 have been noted for cultures independently performed at wave-induced agitation characterized by Re_L equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.

Keywords: Disposable (single-use) bioreactor; Human hematopoietic HL-60 cells; Nonadherent cell propagation; Re number; Wave-type agitation; kLa coefficient.