High NaCl tolerance potential of Bruguiera cylindrica (L.) Blume compromised by mild CuSO4 concentration as evidenced by unique physiochemical features

Abstract

Differential response of Bruguiera cylindrica to individual (CuSO₄) and combined (CuSO₄ NaCl) effect was evaluated. The plantlets were treated with control, 0.15 mM CuSO₄, 400 mM NaCl and 0.15 mM CuSO₄ + 400 mM NaCl. Under combined stress, higher accumulation of Cu in the roots indicate that the roots are the primary site of Cu accumulation and thus the plant perform as an excluder and photosynthetic efficiency reduced drastically and significant enhancement in the superoxide and hydroxyl free radicals which increase membrane lipid peroxidation, leading to cellular damage and destruction. As evidenced from SEM-EDXMA, increase in Cu and Na⁺ levels in xylem and pith regions of leaf and stem and the presence of deeply stained structures, denoting the probable formation of complex containing the metal. Increased CaOx crystal forming cells (crystal idioblasts) reveals the regulation of bioaccumulated Cu and Na⁺ by complexing with CaOx. Thus the study suggested that, 400 mM NaCl and 0.15 mM CuSO₄ treatments does not have negative impact on plant growth, the NaCl tolerance potential compromised in the presence of mild CuSO₄ concentration during combined stress.

Keywords: Bruguiera cylindrica; Combined stress; Crystal idioblast; Electrolytic leakage; MDA; Membrane stability index; Oxidative damage.