Kockdown of ACP5 inhibits hepatocellular carcinoma progression

Rui Shi; Tong Liu; Zirong Liu; Long Yang; Yuan Shi; Yamin Zhang

Kockdown of ACP5 inhibits hepatocellular carcinoma progression

Am J Transl Res. 2020 May 15;12(5):1904-1912. eCollection 2020.

Authors

Rui Shi^{1

2}, Tong Liu³, Zirong Liu^{1

2}, Long Yang^{1

2}, Yuan Shi^{1

2}, Yamin Zhang^{1

2}

Affiliations

¹ Department of Hepatobiliary Surgery, Tianjin First Central Hospital Tianjin, China.
² Liver Transplantation Department, Tianjin First Central Hospital, Key Laboratory for Critical Care Medicine of The Ministry of Health, Tianjin Key Laboratory for Organ Transplantation, Tianjin Clinical Research Center for Organ Transplantation Tianjin 300192, China.
³ Department of General Surgery, Tianjin Medical University General Hospital Tianjin 300052, China.

PMID: 32509186
PMCID: PMC7270020

Abstract

Tartrate-resistant acid phosphatase (ACP5) could regulate cancer cell proliferation; however, its role in hepatocellular carcinoma (HCC) remains largely unknown. Here, we investigated the function of ACP5 in HCC and examined the underlying molecular mechanisms. The expression of ACP5 was evaluated by immunohistochemistry and quantitative reverse transcription and polymerase chain reaction (qRT-PCR) in a series of HCC tissues. The effects of ACP5 silencing on cell proliferation, cell cycle, apoptosis, migration, and invasion of HCC were assessed in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, clonogenic assays, flow cytometry, and transwell assays. The results find that ACP5 is overexpressed in HCC tissues compared with adjacent normal tissues. Knockdown of ACP5 inhibits the proliferation, migration and invasion of HCC cell lines. Furthermore, silencing of ACP5 induces cell cycle G2/M phase arrest and increases apoptosis of HCC cell lines. ACP5 provides potential novel targets for the treatment of HCC.

Keywords: ACP5; Hepatocellular carcinoma; apoptosis; cell cycle; invasion; proliferation.