Structural and functional characterizations of the C-terminal domains of CzcD proteins

Saumya R Udagedara; Daniel M La Porta; Christian Spehar; Ghruta Purohit; Matthew J A Hein; Monique E Fatmous; G Patricia Casas Garcia; Katherine Ganio; Christopher A McDevitt; Megan J Maher

doi:10.1016/j.jinorgbio.2020.111087

Structural and functional characterizations of the C-terminal domains of CzcD proteins

J Inorg Biochem. 2020 Jul:208:111087. doi: 10.1016/j.jinorgbio.2020.111087. Epub 2020 Apr 23.

Affiliations

¹ Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3083, Australia.
² Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne 3000, Australia.
³ Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3083, Australia; School of Chemistry, The Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne 3010, Australia. Electronic address: megan.maher@unimelb.edu.au.

PMID: 32505855
DOI: 10.1016/j.jinorgbio.2020.111087

Abstract

Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist host zinc insults by metal efflux pathways that include cation diffusion facilitator (CDF) proteins. The structural and functional examination of this protein class has been limited, with only the structures of the zinc transporter YiiP proteins from E. coli and Shewanella oneidensis described to date. Here, we determine the metal binding properties, solution quaternary structures and three dimensional architectures of the C-terminal domains of the metal transporter CzcD proteins from Cupriavidus metallidurans, Pseudomonas aeruginosa and Thermotoga maritima. We reveal significant diversity in the metal-binding properties and structures of these proteins and discover a potential novel mechanism for metal-promoted dimerization for the Cupriavidus metallidurans and Pseudomonas aeruginosa proteins.

Keywords: Analytical ultracentrifugation (AUC); Cation diffusion facilitator protein (CDF); CzcD; Isothermal titration calorimetry (ITC); X-ray crystallography; Zinc.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / chemistry*
Bacterial Proteins / chemistry*
Cation Transport Proteins / chemistry*
Protein Domains
Structure-Activity Relationship

Substances

Bacterial Proteins
Cation Transport Proteins