Changes in photosynthesis and respiratory metabolism of maize seedlings growing under low temperature stress may be regulated by arbuscular mycorrhizal fungi

Abstract

Arbuscular mycorrhizal fungi as an important soil microbe have been demonstrated to mitigate the harmful effects of stress on plants. However, little is known about the molecular mechanisms underlying the AM symbiotic response to low temperature. Here, differentially expressed genes (DEGs) in the maize seedlings were identified after inoculating AMF under low temperature conditions. A total of 10,400 DEGs were obtained among four treatments, including non-inoculated AMF under ambient temperature (NMA), inoculated AMF under ambient temperature (MA), non-inoculated with low temperature stress (NML), and inoculated with low temperature stress (ML). The relative expression of 858 genes increased and that of 497 genes decreased in AM plants under low temperature stress. 24 DEGs were identified related to photosynthesis and respiratory metabolism. Among these DEGs, 10 genes were upregulated, and 14 genes were downregulated. The results show that inoculating AMF might decrease the production and transmission of electrons under low temperature, and the cyclic electron flow process in chloroplasts was stimulated to protect plants against low temperature. The fungi also influenced transmission of electrons and production of phosphoric acid in mitochondria in response to low temperature. CO₂ assimilation capacity was affected and the tricarboxylic acid cycle was promoted by the adjustments in the glycolysis, pentose phosphate pathway, gamma-aminobutyric acid shunt pathway, and glyoxylic acid cycle to produce more adenosine triphosphate and raw materials for other metabolic pathways under low temperature. These findings provide new insight into low temperature tolerance induced by AMF, and help identify genes for further investigation and functional analyses.

Keywords: Carbon metabolism; Cold tolerance; Electron transport; RNA sequencing; Zea mays.