A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

Kanako Miyano; Katsuya Ohbuchi; Yuka Sudo; Kouichiro Minami; Toru Yokoyama; Masahiro Yamamoto; Miaki Uzu; Miki Nonaka; Seiji Shiraishi; Hiroaki Murata; Yoshikazu Higami; Yasuhito Uezono

doi:10.1016/j.jphs.2020.05.001

A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy

J Pharmacol Sci. 2020 Aug;143(4):320-324. doi: 10.1016/j.jphs.2020.05.001. Epub 2020 May 11.

Authors

Affiliations

¹ Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
² Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.
³ Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan.
⁴ Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan.
⁵ Department of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan.
⁶ Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.
⁷ Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan. Electronic address: yuezono@ncc.go.jp.

PMID: 32505645
DOI: 10.1016/j.jphs.2020.05.001

Abstract

Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC₅₀) and efficacy (E_max) of these agonists with CellKey™ were almost identical to those made using the traditional Ca²⁺ influx assay in TRPV1- or TRPA1-expressing cells, suggesting that CellKey™ is a simpler and easier means of evaluating TRP activities.

Keywords: CellKey™ system; Cellular dielectric spectroscopy; TRPV1.

MeSH terms

Dielectric Spectroscopy / methods*
HEK293 Cells
Humans
TRPA1 Cation Channel
TRPV Cation Channels
Transient Receptor Potential Channels / metabolism*

Substances

TRPA1 Cation Channel
TRPV Cation Channels
TRPV1 receptor
Transient Receptor Potential Channels