Photosynthesis in a Vanda sp orchid with Photosynthetic Roots

Abstract

PAM fluorometry showed that the orchid Vanda sp (Gaud ex Pfitzers, Vandeae) had photosynthetic electron transport yields in leaves reaching ≈ 0.617 ± 0.262 at midday. Yield decayed exponentially as irradiance increased (Y_½ = 128 ± 12.4 μmol photon m^-2 s^-1). Optimum irradiance (E_opt) for ETR (Photosynthetic Electron Transport Rate) was ≈ 369 ± 23.3 μmol photon m^-2 s^-1; the maximum photosynthetic ETR (ETR_max) (on a Chl a basis) ≈ 97.6 ± 3.76 μmol e^-g^-1 Chl a s^-1. Rapid light curves exhibited classic photoinhibition at high irradiances: Vanda sp is a shade plant. Photosynthetic kinetics was strongly diurnal with minimal E_opt and ETR_max in the early morning, reaching a maximum at midday and decreasing in the afternoon. The aerial roots were normally photosynthetically dormant but rapidly activated when wet (homiochlorophyllous) then becoming dormant again after drying. Wet roots deliberately incubated under moist conditions had photosynthetic light curves comparable to leaves (Y_max ≈0.332, Y½ = ≈ 78.3 ± 27.8 μmol photons m^-2 s^-1, E_opt ≈ 278 μmol photons m^-2 s^-1 and ETR_max ≈ 317 ± 86.9 μmol e^-g^-1 Chl a s^-1): wetting for only 15 min activated photosynthesis. Leaves showed a small degree of diurnal cycling of titratable acid but acid was accumulated in the early morning, not at night, this is a type of CAM-cycling. Titratable acid was low at sunrise (≈ 54.1 μmol H⁺g^-1 FW), but increased until about 9 a.m. (≈ 137 μmol H⁺g^-1 FW) and then gradually decreased over the course of the day.

Keywords: CAM; Homiochlorophylly in roots; PAM; Photosynthesis in roots and leaves; Photosynthetic roots; Vanda orchid.