Genetically encoded biosensors based on innovative scaffolds

Benjamien Moeyaert; Peter Dedecker

doi:10.1016/j.biocel.2020.105761

Genetically encoded biosensors based on innovative scaffolds

Int J Biochem Cell Biol. 2020 Aug:125:105761. doi: 10.1016/j.biocel.2020.105761. Epub 2020 Jun 3.

Authors

Benjamien Moeyaert¹, Peter Dedecker²

Affiliations

¹ Laboratory for Nanobiology, Department of Chemistry, KU Leuven, Celestijnenlaan 200G, 3001 Heverlee, Belgium.
² Laboratory for Nanobiology, Department of Chemistry, KU Leuven, Celestijnenlaan 200G, 3001 Heverlee, Belgium. Electronic address: peter.dedecker@kuleuven.be.

PMID: 32504671
DOI: 10.1016/j.biocel.2020.105761

Abstract

Genetically encoded biosensors are indispensable tools for visualizing the spatiotemporal dynamics of analytes or processes in living cells in vitro and in vivo. Their widespread adaptation has gone hand in hand with the development of sensors for new analytes or processes and improved functionality and robustness. In this review, we highlight some of the recent advances in genetically encoded biosensor development, with a special focus on novel and innovative scaffolds that will lead to new possibilities in the future.

Keywords: Cell imaging; Fluorescent proteins; Genetically encoded biosensors; Protein engineering; Selflabeling enzymes.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Antibodies
Biosensing Techniques / methods*
Calcium / chemistry
Calcium / metabolism
Fluorescence Resonance Energy Transfer / methods*
Luminescent Proteins / chemistry
Neurotransmitter Agents / pharmacology
Protein Engineering / methods*
Receptors, G-Protein-Coupled / chemistry
Receptors, G-Protein-Coupled / drug effects
Receptors, G-Protein-Coupled / genetics*
Signal Transduction / drug effects*

Substances

Antibodies
Luminescent Proteins
Neurotransmitter Agents
Receptors, G-Protein-Coupled
Calcium