CXCL4 suppresses tolerogenic immune signature of monocyte-derived dendritic cells

Eur J Immunol. 2020 Oct;50(10):1598-1601. doi: 10.1002/eji.201948341. Epub 2020 Jul 10.

Abstract

RNA sequencing and DNA methylomic profiling were performed after differentiating monocytes for 6 days into moDCs with/without CXCL4 presence. We show that CXCL4 downregulates genes associated with tolerogenicity in DCs including C1Q. Expression profiles of C1Q genes were negatively correlated with their DNA methylation profiles and with immunogenic genes.

Keywords: C1q; CXCL4; DNA methylation; Monocyte-derived dendritic cells; RNA sequencing.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoimmunity
  • Cell Differentiation
  • Cells, Cultured
  • Complement C1q / genetics
  • Dendritic Cells / immunology*
  • Epigenome
  • Humans
  • Immune Tolerance
  • Monocytes / immunology*
  • Platelet Factor 4 / metabolism*
  • Sequence Analysis, RNA
  • Transcriptome

Substances

  • Platelet Factor 4
  • Complement C1q