Extendable, large-field multi-modal optical imaging system for measuring tissue hemodynamics

Chen Wang; Xiao Chen; Jiachi Hong; Liangwei Meng; Weimin Cheng; Xuan Zhu; Jinling Lu; Pengcheng Li

doi:10.1364/BOE.386197

Extendable, large-field multi-modal optical imaging system for measuring tissue hemodynamics

Biomed Opt Express. 2020 Apr 3;11(5):2339-2351. doi: 10.1364/BOE.386197. eCollection 2020 May 1.

Authors

Chen Wang^{1

2}, Xiao Chen^{1

2}, Jiachi Hong^{1

2}, Liangwei Meng^{1

2}, Weimin Cheng^{1

2}, Xuan Zhu^{1

2}, Jinling Lu^{1

2}, Pengcheng Li^{1

2

3}

Affiliations

¹ Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
² MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
³ HUST-Suzhou Institute for Brainsmatics, Suzhou 215125, China.

Abstract

Simultaneous imaging of multiple hemodynamic parameters helps to evaluate the physiological and pathological status of biological tissue. To achieve multimodal hemodynamics imaging with a large field of view, an infinite conjugate relay lens system compatible with the standard C-Mount camera lens is designed to adapt one camera lens with multiple CCD/CMOS cameras for simultaneously multi-wavelength imaging. Using this relay lens system, dual wavelength reflectance imaging and laser speckle contrast imaging were combined to simultaneously detect the changes in blood flow, oxygenation, and hemoglobin concentrations. To improve the accuracy of hemoglobin concentration measurement with an LED illumination source, an integral algorithm is proposed that accounts for the dependence of differential pathlength factors (DPF) on hemoglobin concentrations and the integral effect of both the emission spectrum of the light source and the spectrum response of the detector. The imaging system is validated by both phantom and in vivo experiments, including the arterial occlusion, and the detection of blood volume pulse (BVP) and blood flow pulse (BFP) signal in human subjects. The system helps in the exploration of macroscopic tissue hemodynamics.