Porous graphene based electrochemical immunosensor using Cu3(BTC)2 metal-organic framework as nonenzymatic label

Talanta. 2020 Sep 1:217:121042. doi: 10.1016/j.talanta.2020.121042. Epub 2020 Apr 14.

Abstract

An electrochemical immunosensor for highly sensitive detection of cancer biomarkers has been developed based on the combination of a sensing platform of polydopamine modified porous graphene and a nonenzymatic label of metal-organic framework (MOF) conjugated secondary antibody. This approach achieves a wide range of linear response from 0.1 to 10 ng/mL, low detection limit of 0.025 ng/mL (at a signal to noise ratio of 3), good reproducibility and selectivity for the detection of prostate specific antigen (PSA) as a model analyte. The high performance of the immunosensor is attributed to the high surface area from porous graphene and the strong adhesion of polydopamine, allowing a high load of the primary antibody of PSA, as well as the highly electrocatalytic activity of the Cu3(BTC)2 (BTC = benzene-1,3,5-tricarboxylic acid) MOF toward H2O2 to provide greatly amplified sensitivity. In this respect, the MOF-based nonenzymatic label shows promising application for the point-of-care detection of different cancer biomarkers in clinical diagnostics.

Keywords: Cancer biomarkers; Graphene; Immunosensors; Metal-organic frameworks; Nonenzymatic labels.

MeSH terms

  • Antibodies / chemistry
  • Biomarkers, Tumor / analysis*
  • Biosensing Techniques*
  • Electrochemical Techniques*
  • Graphite / chemistry
  • Humans
  • Hydrogen Peroxide / chemistry
  • Immunoassay*
  • Metal-Organic Frameworks / chemistry*
  • Molecular Structure
  • Particle Size
  • Porosity
  • Prostate-Specific Antigen / analysis*
  • Surface Properties

Substances

  • Antibodies
  • Biomarkers, Tumor
  • Metal-Organic Frameworks
  • bis(1,3,5-benzenetricarboxylate)tricopper(II)
  • Graphite
  • Hydrogen Peroxide
  • Prostate-Specific Antigen