Fluorometric detection of dopamine based on 3-aminophenylboronic acid-functionalized AgInZnS QDs and cells imaging

Yibing Liu; Xu He; Pinyi Ma; Yibing Huang; Xiaolei Li; Ying Sun; Xinghua Wang; Daqian Song

doi:10.1016/j.talanta.2020.121081

Fluorometric detection of dopamine based on 3-aminophenylboronic acid-functionalized AgInZnS QDs and cells imaging

Talanta. 2020 Sep 1:217:121081. doi: 10.1016/j.talanta.2020.121081. Epub 2020 Apr 25.

Authors

Yibing Liu¹, Xu He², Pinyi Ma³, Yibing Huang⁴, Xiaolei Li⁵, Ying Sun¹, Xinghua Wang¹, Daqian Song⁶

Affiliations

¹ College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
² College of Life Sciences, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
³ College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, China. Electronic address: mapiny@jlu.edu.cn.
⁴ College of Life Sciences, Jilin University, Qianjin Street 2699, Changchun, 130012, China. Electronic address: huangyibing@jlu.edu.cn.
⁵ School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Liaocheng University, Liaocheng, 252059, China.
⁶ College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, China. Electronic address: songdq@jlu.edu.cn.

PMID: 32498860
DOI: 10.1016/j.talanta.2020.121081

Abstract

Herein, cysteine capped AgInZnS QDs (Cys-AIZS QDs) with a large stoke shift and excellent biocompatibility were synthesized by a one-step aqueous method, followed by modified with 3-aminophenylboronic acid (APBA). Dopamine (DA) as an important neurotransmitter in brain can lead to significantly decrease in the fluorescence intensity of 3-aminophenylboronic acid-functionalized Cys-AIZS QDs (APBA-AIZS QDs) in a large concentration range of 1.5-900 μM. Good linearity can be obtained in the range of 15-120 μM, with a limit of detection (LOD) of 0.65 μM. Moreover, Cys-AIZS QDs and APBA-AIZS QDs were applied to living cells imaging, and Cys-AIZS QDs were applied to the co-localization with lysosomes, indicative of the feasibility of intracellular detection.

Keywords: 3-Aminophenylboronic acid; AgInZnS QDs; Cells imaging; Dopamine; Lysosomes co-localization.

MeSH terms

Adult
Biosensing Techniques
Boronic Acids / chemistry*
Dopamine / analysis*
Fluorometry*
Healthy Volunteers
Humans
Indium / chemistry
Optical Imaging*
Particle Size
Quantum Dots / chemistry*
Silver / chemistry
Sulfur / chemistry
Surface Properties
Zinc / chemistry

Substances

Boronic Acids
Indium
3-aminobenzeneboronic acid
Silver
Sulfur
Zinc
Dopamine