A new cypovirus from the Japanese peppered moth, Biston robustus

Jun Takatsuka

doi:10.1016/j.jip.2020.107417

A new cypovirus from the Japanese peppered moth, Biston robustus

J Invertebr Pathol. 2020 Jul:174:107417. doi: 10.1016/j.jip.2020.107417. Epub 2020 Jun 1.

Author

Jun Takatsuka¹

Affiliation

¹ Forestry and Forest Products Research Institute, Forest Research and Management Organization, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan. Electronic address: junsan@ffpri.affrc.go.jp.

PMID: 32497527
DOI: 10.1016/j.jip.2020.107417

Abstract

A cypovirus was isolated from larvae of the Japanese peppered moth, Biston robustus. The viral genome is 23,954 bp comprising 10 segmented double-stranded RNAs with a new electropherotype among cypoviruses. Each segment encodes one putative protein and has non-coding regions that contain conserved sequences at their 5' and 3' termini, 5'-AGAA(U/A)U-3' and 5'-UGC-3', respectively. Seven proteins encoded in the genome are homologous to those of other cypoviruses at a cut-off E-value of 1 × 10^-5. The maximal sequence identities of these proteins with cypovirus homologs are 24.30%-39.40%. These results indicate that the virus isolated is a novel cypovirus; herein designated as Biston robustus cypovirus 24 (BrCPV-24). This newly isolated BrCPV-24 infects the larvae of the silkworm Bombyx mori.

Keywords: Biston robustus; Cypovirus; Geometridae; Reovirus; Spinareovirinae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome, Viral*
Japan
Larva / growth & development
Larva / virology
Moths / growth & development
Moths / virology*
Phylogeny
Reoviridae / classification*
Reoviridae / genetics
Reoviridae / isolation & purification