Serum Protein Corona Abolishes Changes in the Expression of Proinflammatory Genes Induced by Quantum Dots in Human Blood Mononuclear Cell

D V Novikov; S G Selivanova; N V Krasnogorova; E N Gorshkova; S N Pleskova; V V Novikov; A V Karaulov

doi:10.1007/s10517-020-04832-7

Serum Protein Corona Abolishes Changes in the Expression of Proinflammatory Genes Induced by Quantum Dots in Human Blood Mononuclear Cell

Bull Exp Biol Med. 2020 May;169(1):95-99. doi: 10.1007/s10517-020-04832-7. Epub 2020 Jun 3.

Authors

D V Novikov^{1

2}, S G Selivanova³, N V Krasnogorova⁴, E N Gorshkova⁴, S N Pleskova^{4

5}, V V Novikov^{4

3}, A V Karaulov⁶

Affiliations

¹ N. I. Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia. novikov.dv75@mail.ru.
² I. N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russia. novikov.dv75@mail.ru.
³ I. N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Federal Service for Supervision of Consumer Rights Protection and Human Welfare, Nizhny Novgorod, Russia.
⁴ N. I. Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia.
⁵ R. E. Alekseev Nizhny Novgorod State Technical University, Nizhny Novgorod, Russia.
⁶ I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.

PMID: 32495165
DOI: 10.1007/s10517-020-04832-7

Abstract

We studied changes in the transcription of genes encoding cytokines (TNF, IL-6, IL-10, and IL-32), cell activation markers (ICAM1, CD38, Fas, and FCGRIII), ROS production catalyst (NOX2), autophagy (Beclin 1, LC3B, and p62) and apoptosis (BAX, BCL2) regulators in peripheral blood mononuclear cells upon contact with quantum dots CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP. Up-regulation of TNF, ICAM1, Fas, p62 mRNA and down-regulation of the FCGRIII and NOX2 mRNA in response to the presence of quantum dots were revealed. The formation of serum protein corona on the surface of quantum dots abolished this effect.

Keywords: mononuclear cells; proinflammatory genes; protein corona; quantum dots.

MeSH terms

Adult
Apoptosis / drug effects
Autophagy / drug effects
Cadmium Compounds / pharmacology
Cadmium Compounds / toxicity
Cytokines / metabolism
Gene Expression / drug effects
Humans
Inflammation / genetics*
Inflammation Mediators / metabolism
Leukocytes, Mononuclear / drug effects*
Leukocytes, Mononuclear / immunology
Leukocytes, Mononuclear / metabolism
Protein Corona / chemistry*
Quantum Dots* / chemistry
Quantum Dots* / toxicity
Reactive Oxygen Species / metabolism
Selenium Compounds / pharmacology
Selenium Compounds / toxicity
Zinc Compounds / pharmacology
Zinc Compounds / toxicity

Substances

Cadmium Compounds
Cytokines
Inflammation Mediators
Protein Corona
Reactive Oxygen Species
Selenium Compounds
Zinc Compounds