Super-resolution nanoscopy by coherent control on nanoparticle emission

Congyue Liu; Wei Liu; Shufeng Wang; Hongjia Li; Zhilong Lv; Fa Zhang; Donghui Zhang; Junlin Teng; Tao Zheng; Donghai Li; Mingshu Zhang; Pingyong Xu; Qihuang Gong

doi:10.1126/sciadv.aaw6579

Super-resolution nanoscopy by coherent control on nanoparticle emission

Sci Adv. 2020 Apr 17;6(16):eaaw6579. doi: 10.1126/sciadv.aaw6579. eCollection 2020 Apr.

Authors

Congyue Liu¹, Wei Liu¹, Shufeng Wang^{1

2

3}, Hongjia Li^{4

5}, Zhilong Lv^{4

5}, Fa Zhang⁴, Donghui Zhang⁶, Junlin Teng⁶, Tao Zheng⁷, Donghai Li⁸, Mingshu Zhang⁹, Pingyong Xu^{9

10}, Qihuang Gong^{1

2

3}

Affiliations

¹ State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871, China.
² Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, Shanxi, China.
³ Frontiers Science Center for Nano-optoelectronics, Peking University, Collaborative Innovation Center of Quantum Matter, Beijing 100871, China.
⁴ High Performance Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, China.
⁵ University of Chinese Academy of Sciences, Beijing, China.
⁶ Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China.
⁷ Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
⁸ Institut für Physikalische und Theoretische Chemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
⁹ Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
¹⁰ College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100101, China.

Abstract

Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ~50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.

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