This paper presents the design of a wireless portable and multichannel potentiostat for remote monitoring in enclosed environments for long-time applications. In this paper, the proposed potentiostat is tested for monitoring the glucose concentration during the fermentation of yeast in real time for more than 24 h. The potentiostat is powered by a USB-connected battery and operated through a Bluetooth using a LabVIEW designed data monitoring and control panel. The potentiostat is capable of performing cyclic voltammetry or chronoamperometry on six biosensors simultaneously and gives the real-time response using Bluetooth connection. The potentiostat has a common counter electrode and reference electrode connection to all biosensors and independent working electrodes for all biosensors. The potentiostat was tested and validated by comparing the results obtained by a commercial potentiostat. The tests performed for monitoring the glucose concentration during the fermentation process showed a current detection limit of 180 nA and reported a standard deviation of ±2% for anodic and cathodic current peaks for cyclic voltammetry measurements when compared with the commercially available device. This study enables the novel method of monitoring the fermentation process wirelessly for days.