Multi-scale data-driven engineering for biosynthetic titer improvement

Zhixing Cao; Jiaming Yu; Weishan Wang; Hongzhong Lu; Xuekui Xia; Hui Xu; Xiuliang Yang; Lianqun Bao; Qing Zhang; Huifeng Wang; Siliang Zhang; Lixin Zhang

doi:10.1016/j.copbio.2020.04.002

Multi-scale data-driven engineering for biosynthetic titer improvement

Curr Opin Biotechnol. 2020 Oct:65:205-212. doi: 10.1016/j.copbio.2020.04.002. Epub 2020 May 30.

Authors

Zhixing Cao¹, Jiaming Yu², Weishan Wang³, Hongzhong Lu⁴, Xuekui Xia⁵, Hui Xu⁶, Xiuliang Yang⁷, Lianqun Bao⁸, Qing Zhang⁹, Huifeng Wang¹⁰, Siliang Zhang², Lixin Zhang¹¹

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; MOE Key Laboratory of Advanced Control and Optimization for Chemical Processes, East China University of Science and Technology, Shanghai 200237, China. Electronic address: zcao@ecust.edu.cn.
² State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
³ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE412 96 Gothenburg, Sweden.
⁵ Key Biosensor Laboratory of Shandong Province, Biology Institute, QiluUniversity of Technology (Shandong Academy of Sciences), Jinan, 250013, China.
⁶ Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
⁷ Shandong Jincheng Bio-Pharmaceutical Co., Ltd., No. 117 Qixing River Road, Zibo 255130, Shandong, China.
⁸ Shijiazhuang XingbaiBioengineering Co., Ltd, Shijiazhuang 050000, China.
⁹ Inner Mongolia New VeyongBiochemical Co., Ltd, Dalad Banner 014300, China.
¹⁰ MOE Key Laboratory of Advanced Control and Optimization for Chemical Processes, East China University of Science and Technology, Shanghai 200237, China. Electronic address: whuifeng@ecust.edu.cn.
¹¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: lxzhang@ecust.edu.cn.

PMID: 32485576
DOI: 10.1016/j.copbio.2020.04.002

Abstract

Industrial biosynthesis is a very complex process which depends on a range of different factors, from intracellular genes and metabolites, to extracellular culturing conditions and bioreactor engineering. The identification of species that improve the titer of some reaction is akin to the task of finding a needle in a haystack. This review aims to summarize state-of-the-art biosynthesis titer improvement on different scales separately, particularly regarding the advancement of metabolic pathway rewiring and data-driven process optimization and control. By integrating multi-scale data and establishing a mathematical replica of a real biosynthesis, more refined quantitative insights can be gained for achieving a higher titer than ever.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bioreactors*
Biosynthetic Pathways / genetics
Fermentation
Metabolic Engineering
Metabolic Networks and Pathways*