Tree age-related effects on sun acclimated leaves in a chronosequence of beech (Fagus sylvatica) stands

Funct Plant Biol. 2012 May;39(4):323-331. doi: 10.1071/FP11248.

Abstract

The assessment of the effect of tree age on leaves is usually limited by the difficulty of sampling sun leaves from tall ageing trees. In this study, we investigated tree age-related effects on sun leaves in a chronosequence of beech (Fagus sylvatica L.) stands. The effects of stand age on leaf mass to area ratio (LMA), chlorophyll (Chl), epidermal polyphenols (EPhen), nitrogen and carbon contents in sun leaves were investigated in 17 even-aged stands distributed into six age classes (14-175 years old). Chl and EPhen were assessed in vivo with SPAD and Dualex portable leaf-clips respectively. Leaves were sampled by shooting and sun leaves were identified based on criteria obtained from a vertical profile of the ratio abaxial vs adaxial EPhen across the canopy. Sun leaves were characterised by a high and similar adaxial and abaxial EPhen contents, high LMA value and low mass-based Chl content. These sun leaf characteristics, together with leaf nitrogen and carbon contents, were not significantly affected by stand age. Along the chronosequence, beech trees invested a stable fraction of leaf mass into nitrogen, carbon, Chl and EPhen with decreasing leaf size, i.e. dry mass and area.