Mobile healthcare system based on the combination of a lateral flow pad and smartphone for rapid detection of uric acid in whole blood

Nan-Si Li; Ying-Tzu Chen; Ying-Pei Hsu; Hao-Han Pang; Chiung-Yin Huang; Yow-Ling Shiue; Kuo-Chen Wei; Hung-Wei Yang

doi:10.1016/j.bios.2020.112309

Mobile healthcare system based on the combination of a lateral flow pad and smartphone for rapid detection of uric acid in whole blood

Biosens Bioelectron. 2020 Sep 15:164:112309. doi: 10.1016/j.bios.2020.112309. Epub 2020 May 21.

Authors

Nan-Si Li¹, Ying-Tzu Chen², Ying-Pei Hsu³, Hao-Han Pang¹, Chiung-Yin Huang⁴, Yow-Ling Shiue⁵, Kuo-Chen Wei⁶, Hung-Wei Yang⁷

Affiliations

¹ Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
² Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
³ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Department of Materials and Optoelectronic Science, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
⁴ Department of Neurosurgery, New Taipei Municipal TuCheng Hospital, Chang Gung Memorial Hospital and Chang Gung University, New Taipei City, 23652, Taiwan.
⁵ Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
⁶ Department of Neurosurgery, New Taipei Municipal TuCheng Hospital, Chang Gung Memorial Hospital and Chang Gung University, New Taipei City, 23652, Taiwan. Electronic address: kuochenwei@cgmh.org.tw.
⁷ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan. Electronic address: howardyang@mail.nsysu.edu.tw.

PMID: 32479340
DOI: 10.1016/j.bios.2020.112309

Abstract

Excessive production of uric acid (UA) in blood may lead to gout, hyperuricaemia and kidney disorder; thus, a fast, simple and reliable biosensor is needed to routinely determine the UA concentration in blood without pretreatment. The purpose of this study was to develop a mobile healthcare (mHealth) system using a drop of blood, which comprised a lateral flow pad (LFP), mesoporous Prussian blue nanoparticles (MPBs) as artificial nanozymes and auto-calculation software for on-site determination of UA in blood and data management. A standard curve was found to be linear in the range of 1.5-8.5 mg/dL UA, and convenience, cloud computing and personal information management were simultaneously achieved for the proposed mHealth system. Our mHealth system appropriately met the requirements of application in patients' homes, with the potential of real-time monitoring by their primary care physicians (PCPs).

Keywords: Colorimetric biosensor; Nanozyme; Smartphone reader; Uric acid; mHealth.

MeSH terms

Biosensing Techniques*
Delivery of Health Care*
Gout* / diagnosis
Humans
Smartphone*
Uric Acid / blood

Substances

Uric Acid