Abstract
The lysosomal protease cathepsin B recognizes defined, short peptide sequences, providing means for effective, targeted drug release. Here, we show that the introduction of a coordination complex adjacent to the cleavage sequence allows us to tune enzymatic cleavage rate by varying the complexed, trivalent metal ion.
MeSH terms
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Amino Acid Sequence
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Cathepsin B / metabolism*
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Cations / chemistry
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Chelating Agents / chemistry
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Coordination Complexes / chemistry*
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Coordination Complexes / metabolism*
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Enzyme Activation
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Kinetics
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Lysosomes / chemistry
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Metals / chemistry
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Molecular Conformation
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Structure-Activity Relationship
Substances
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Cations
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Chelating Agents
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Coordination Complexes
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Metals
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Cathepsin B