Identifying Lipid Metabolites Influenced by Oleic Acid Administration Using High-Performance Liquid Chromatography-Mass Spectrometry-Based Lipidomics

Chao Xu; Dan Song; Askild L Holck; Youyou Zhou; Rong Liu

doi:10.1021/acsomega.9b04402

Identifying Lipid Metabolites Influenced by Oleic Acid Administration Using High-Performance Liquid Chromatography-Mass Spectrometry-Based Lipidomics

ACS Omega. 2020 Apr 29;5(20):11314-11323. doi: 10.1021/acsomega.9b04402. eCollection 2020 May 26.

Authors

Chao Xu¹, Dan Song¹, Askild L Holck², Youyou Zhou¹, Rong Liu^{1

3

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Affiliations

¹ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
² NOFIMA - Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210, N-1431 Aas, Norway.
³ National Center for International Research on Animal Gut Nutrition, Nanjing 210095, China.
⁴ Jiangsu Collaborative Innovation Center of Meat Production and Processing, Nanjing 210095, China.

Abstract

Oleic acid (OA), one of the most important monounsaturated fatty acids, possesses protective properties against chronic liver disease (CLD) development, but the underlying metabolic metabolism remains unknown. HPLC-MS-based lipidomics was utilized to identify and quantify the endogenously altered lipid metabolites when hepatocytes were exposed to OA administration. The identified lipids could be grouped into 22 lipid classes; of which, 10 classes were significantly influenced by the OA treatment: lysophosphatidylcholine (LPC), phosphatidylglycerol (PG), ceramides (Cer), hexosylceramides (Hex1Cer), dihexosylceramides (Hex2Cer), cholesterol ester (ChE), and coenzyme (Co) were decreased, while diglyceride (DG), triglyceride (TG), and acyl carnitine (AcCa) were increased. In addition, as the variable importance in projection (VIP) list (VIP > 1.0 and P < 0.05) showed, 478 lipid species showed significant difference with OA administration, and these molecules could be potential biomarkers in conjunction with OA administration. In summary, our results provided a novel perspective to understand the influences of OA administration by investigating endogenous altered levels of lipid metabolites via lipidomics.