Role of Blood Oxygen Saturation During Post-Natal Human Cardiomyocyte Cell Cycle Activities

Lincai Ye; Lisheng Qiu; Bei Feng; Chuan Jiang; Yanhui Huang; Haibo Zhang; Hao Zhang; Haifa Hong; Jinfen Liu

doi:10.1016/j.jacbts.2020.02.008

Role of Blood Oxygen Saturation During Post-Natal Human Cardiomyocyte Cell Cycle Activities

JACC Basic Transl Sci. 2020 Apr 22;5(5):447-460. doi: 10.1016/j.jacbts.2020.02.008. eCollection 2020 May.

Authors

Lincai Ye^{1

2

3}, Lisheng Qiu¹, Bei Feng^{1

2

3}, Chuan Jiang^{1

2}, Yanhui Huang⁴, Haibo Zhang¹, Hao Zhang^{1

2}, Haifa Hong^{1

2}, Jinfen Liu²

Affiliations

¹ Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.
² Shanghai Institute for Pediatric Congenital Heart Diseases, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.
³ Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.
⁴ Department of Anesthesiology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Abstract

Blood oxygen saturation (SaO₂) is one of the most important environmental factors in clinical heart protection. This study used human heart samples and human induced pluripotent stem cell-cardiomyocytes (iPSC-CMs) to assess how SaO₂ affects human CM cell cycle activities. The results showed that there were significantly more cell cycle markers in the moderate hypoxia group (SaO₂: 75% to 85%) than in the other 2 groups (SaO₂ <75% or >85%). In iPSC-CMs 15% and 10% oxygen (O₂) treatment increased cell cycle markers, whereas 5% and rapid change of O₂ decreased the markers. Moderate hypoxia is beneficial to the cell cycle activities of post-natal human CMs.

Keywords: CHD, congenital heart disease; CM, cardiomyocytes; IF, immunofluorescence; LV, lentivirus; O2, oxygen; SaO2, blood oxygen saturation; TOF, tetralogy of Fallot; YAP1, yes-associated protein 1; blood oxygen saturation; cardiomyocyte; congenital heart disease; iPSC, induced pluripotent stem cell; pATM, phosphorylated ataxia telangiectasia mutated; pHH3, phospho-histone H3; pediatric patients; proliferation; qPCR, quantitative polymerase chain reaction; sh, short hairpin.