Simple Recommendations for Improving Efficiency in Generating Genome-Edited Mice

O A Averina; M Y Vysokikh; O A Permyakov; P V Sergiev

doi:10.32607/actanaturae.10937

Simple Recommendations for Improving Efficiency in Generating Genome-Edited Mice

Acta Naturae. 2020 Jan-Mar;12(1):42-50. doi: 10.32607/actanaturae.10937.

Authors

O A Averina^{1

2}, M Y Vysokikh², O A Permyakov¹, P V Sergiev^{1

3}

Affiliations

¹ Institute of functional genomics, Lomonosov Moscow State University, Moscow, 119991 Russia.
² Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991 Russia.
³ Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991 Russia.

Abstract

The generation of transgenic model organisms (primarily mice) is an integral part of modern fundamental and applied research. Simple techniques based on the biology of these laboratory rodents can often increase efficiency when generating genome-edited mouse strains. In this study, we share our three years of experience in the optimization of mouse genome editing based on microinjection of CRISPR/Cas9 components into ca. 10,000 zygotes. We tested a number of techniques meant to improve efficiency in generating knockout mice, such as optimization of the superovulation method and choosing the optimal mouse strains to be used as zygote donors and foster mothers. The presented results might be useful to laboratories aiming to quickly and efficiently create new mouse strains with tailored genome editing.

Keywords: genome editing; mouse foster mothers; mouse zygote donors; superovulation; transgenic mice.