Abstract
We report a novel scaffold for clustering and oriented immobilization of human IgG1 Fc-fused lectins on biosensors without chemical modifications. This approach uses a bio-nanocapsule (BNC) displaying a tandem form of IgG Fc-binding Z domains derived from Staphylococcus aureus protein A (ZZ-BNC). Incorporating ZZ-BNC effectively increased both the sensitivity and sugar chain-binding capacity compared with the condition without ZZ-BNC.
Keywords:
Fc-fused lectin; Sugar chain; bio-nanocapsule; biosensor; oriented immobilization.
MeSH terms
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Biosensing Techniques / methods*
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Humans
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Immobilized Proteins / chemistry
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Immobilized Proteins / metabolism*
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Immunoglobulin Fc Fragments / genetics*
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Immunoglobulin G / immunology
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Lectins / chemistry
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Lectins / metabolism*
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Protein Domains
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
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Staphylococcal Protein A / chemistry
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Sugars / analysis*
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Sugars / chemistry
Substances
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Immobilized Proteins
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Immunoglobulin Fc Fragments
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Immunoglobulin G
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Lectins
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Recombinant Fusion Proteins
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Staphylococcal Protein A
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Sugars