Chemical and Microstructural Characterization of pH and [Ca2+] Dependent Sol-Gel Transitions in Mucin Biopolymer

Austin Curnutt; Kaylee Smith; Emily Darrow; Keisha B Walters

doi:10.1038/s41598-020-65392-4

Chemical and Microstructural Characterization of pH and [Ca²⁺] Dependent Sol-Gel Transitions in Mucin Biopolymer

Sci Rep. 2020 May 29;10(1):8760. doi: 10.1038/s41598-020-65392-4.

Authors

Austin Curnutt¹, Kaylee Smith¹, Emily Darrow¹, Keisha B Walters²

Affiliations

¹ School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, Oklahoma, 73019, USA.
² School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, Oklahoma, 73019, USA. keisha.walters@ou.edu.

Abstract

Mucus is responsible for controlling transport and barrier function in biological systems, and its properties can be significantly affected by compositional and environmental changes. In this study, the impacts of pH and CaCl₂ were examined on the solution-to-gel transition of mucin, the primary structural component of mucus. Microscale structural changes were correlated with macroscale viscoelastic behavior as a function of pH and calcium addition using rheology, dynamic light scattering, zeta potential, surface tension, and FTIR spectroscopic characterization. Mucin solutions transitioned from solution to gel behavior between pH 4-5 and correspondingly displayed a more than ten-fold increase in viscoelastic moduli. Addition of CaCl₂ increased the sol-gel transition pH value to ca. 6, with a twofold increase in loss moduli at low frequencies and ten-fold increase in storage modulus. Changing the ionic conditions-specifically [H⁺] and [Ca²⁺] -modulated the sol-gel transition pH, isoelectric point, and viscoelastic properties due to reversible conformational changes with mucin forming a network structure via non-covalent cross-links between mucin chains.