Lancemaside A Isolated from the Root of Codonopsis lanceolata Inhibits Ovarian Cancer Cell Invasion via the Reactive Oxygen Species (ROS)-Mediated p38 Pathway

Ji-Hye Ahn; Dae Sik Jang; Jung-Hye Choi

doi:10.1142/S0192415X20500494

Lancemaside A Isolated from the Root of Codonopsis lanceolata Inhibits Ovarian Cancer Cell Invasion via the Reactive Oxygen Species (ROS)-Mediated p38 Pathway

Am J Chin Med. 2020;48(4):1021-1034. doi: 10.1142/S0192415X20500494. Epub 2020 May 29.

Authors

Ji-Hye Ahn^{1

2

3}, Dae Sik Jang⁴, Jung-Hye Choi^{1

3}

Affiliations

¹ Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447, South Korea.
² Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Pinn Hall 1232, Charlottesville, VA, 22908, USA.
³ Department of Oriental Pharmacy, College of Pharmacy, Woosuk University, Jeonbuk 55338, South Korea.
⁴ Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, South Korea.

PMID: 32471314
DOI: 10.1142/S0192415X20500494

Abstract

Codonopsis lanceolata roots have been widely used in Korean cuisine and traditional medicine. This study aimed to investigate the antimetastatic effects of lancemaside A, a major triterpenoid saponin, isolated from the roots of C. lanceolata, in human ovarian cancer cells. Lancemaside A significantly suppressed the migration and invasion and the expression of matrix metalloproteinases (MMPs)-2 and -9 in ovarian cancer A2780 and SKOV3 cells. Treatment with lancemaside A generated reactive oxygen species (ROS) in ovarian cancer cells. However, treatment with anti-oxidant N-acetyl-L-cysteine (NAC) significantly negated the anti-invasive activity of lancemaside A. Additionally, lancemaside A activated p38 MAP kinase, which is mediated by ROS generation. This is the first study, to our knowledge, to reveal that lancemaside A isolated from the roots of C. lanceolata exerts antimetastatic activity through inhibition of MMP expression and cancer cell invasion via activation of the ROS-mediated p38 pathway.

Keywords: Codonopsis lanceolata; Invasion; Lancemaside A; Ovarian Cancer; Reactive Oxygen Species; p38.

MeSH terms

Antineoplastic Agents, Phytogenic
Cell Line, Tumor
Codonopsis / chemistry*
Female
Gene Expression
Humans
MAP Kinase Signaling System / drug effects*
MAP Kinase Signaling System / genetics*
Neoplasm Invasiveness / genetics*
Neoplasm Invasiveness / pathology*
Ovarian Neoplasms / pathology*
Plant Roots / chemistry*
Reactive Oxygen Species / metabolism*
Saponins / isolation & purification
Saponins / pharmacology*
p38 Mitogen-Activated Protein Kinases / genetics
p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

Antineoplastic Agents, Phytogenic
Reactive Oxygen Species
Saponins
lancemaside A
p38 Mitogen-Activated Protein Kinases